Sygnature Discovery > Drug Discovery > Protein Science & Structural Biology > Biophysical Characterisation

Biophysical Characterisation

At Sygnature Discovery, we recognise that biophysics is a valuable component of drug discovery programs. We have invested in the establishment of scientific excellence and industry leading technology to grow our biophysics capability and to imbed the value of its use across our scientific teams.

Biophysics

Our biophysics platform provides techniques that can deliver hit finding approaches for fragment-based lead discovery and a full mechanistic, structural, kinetic, and thermodynamic profile for your novel compounds.

We have dedicated biophysicists who can support your integrated drug discovery program with a suite of high-end technology platforms that can deliver a complete biophysical assessment of your compounds of interest.

In addition to the range of in-house biophysics platforms, Sygnature has selected trusted strategic alliance partners to augment our biophysics capabilities, providing you with access to discovery excellence.

 

 

Biophysical Characterisation Services

Surface Plasmon Resonance (SPR)

Our biophysicists are proficient using state-of-the-art equipment to carry out label-free SPR assays, which measure compound, fragment or larger species (e.g., protein-protein) binding in real-time to generate accurate kinetic and binding mechanism information about a wide range of drug targets.

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Fluorescent Thermal Shift Assays (FTSA)

Obtain high-quality insights into the thermal unfolding, stability, and ligand effects of your protein target with our FTSA (Differential Scanning Fluorimetry or DSF). Discover valuable information to refine the binding mechanism of action for your drug target.

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Nuclear Magnetic Resonance (NMR)

Leveraging cutting-edge NMR instruments, Sygnature Discovery scientists accurately describe compound, fragment, and protein interactions without the need for labels, preserving their integrity.

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Flow Induced Dispersion Analysis (FIDA)

FIDA allows the rapid measurement of protein size in solution. This can be achieved with or without protein labelling and requires only a small amount of sample.

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Protein Mass Spectrometry

We have a Sciex X500B QTOF mass spectrometer which we use for quality control of every batch of protein we produce.

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X-Ray Crystallography (XRC)

Enhance your discovery program with X-ray crystallography (XRC) - precise mapping of interaction sites in lead protein molecules.

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Isothermal Titration Calorimetry (ITC)

Gain valuable insights into the binding mechanism of novel compounds during lead identification through our comprehensive thermodynamic and kinetic profiling services, utilising ITC analysis to determine crucial parameters for your drug candidate research.

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Microscale Thermophoresis (MST)

Leveraging a robust homogeneous assay solution-based technology, Sygnature Discovery analyses precise thermal gradient effects on molecular properties to assess ligand binding interactions. Our skilled biophysicists provide valuable insights that complement SPR findings.

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Dynamic Light Scattering (DLS)

Our biophysicists can assess the aggregation behaviour of your compounds, targets and complexes within solution using DLS techniques. This can assist you in the characterisation of lead compounds and in the development of drug delivery systems or biologic therapeutics.

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