Cell Based Assays

Cellular Interaction and Co Culture Assay Systems

Assay formats that culture two or more cell types together can be used to provide a more accurate model of the target tissue environmental context. Our scientists are proficient at generating complex in vitro co-culture assays to study cellular Interaction for your particular target area, including those for vascular branching angiogenesis assays, and phagocytotic assays.

Cell Proliferation and Viability Assay

Cell health in response to drug treatment is an important factor in any drug discovery project. Our scientists routinely use assays to profile the cytostatic or cytotoxic nature of compounds using assays to detect alterations in key biomarkers involved in these processes such as ATP, caspases or DNA replication, as well as examining cell cycle progression and mitochondrial integrity.

Cell Population Analysis

Harness the power of flow cytometry and high-content imaging for comprehensive heterogeneous cell population analysis. Assess cell cycle stages and biomarkers in response to target treatment, gaining valuable insights.

High Content Imaging and 3D Cultures

Capture high-quality images using our advanced IXM-C imaging system. Our versatile assay formats cater to various stages of the drug discovery process, including 2D and 3D cultures. Leveraging 3D cell cultures enables retention of tissue architecture and cell-matrix interactions, providing highly relevant in vitro models for studying drug efficacy and safety.

Chemotaxic

The stimulus-induced migration of cells is co-ordinated tightly by complex signalling networks, and development of drugs to target these pathways are important in many therapeutic areas including tumour metastasis and wound healing. We have established chemotactic assays to quantify migrated cells in response to drug treatments.

Fibrosis Assays

Fibrosis is an abnormal accumulation of excess extracellular matrix (ECM) components around damaged tissue and can cause excessive scarring, changes in tissue structure leading to organ disease and failure. Sygnature Discovery has developed in vitro organ-specific fibrosis models using confocal microscopy, allowing high-throughput screening of potential anti-fibrotic candidates.

Intracellular Signalling

Discover how intracellular signalling influences cell function, survival, and differentiation. Our varied assay portfolio elucidates the impact of your target on cellular signalling networks.

Phenotypic Screening

Our expert bio-scientists here at Sygnature Discovery, excel in developing phenotypic screens across various cell types, including primary cells and disease-relevant models. Explore our proficiency in assays for primary cells and immortalised lines.

Protein Expression

Analysing the expression of genes and their protein products are a vital means to understand cell functionality in response to drug exposure. Our scientists have expertise in both quantitative PCR assays and detection of analytes in single (e.g. western blot) and multiplex (e.g. AlphaLISA, Luminex and Flow Cytometry) format to evaluate the effects of your target on various signalling and expression pathways.