Protein Science

We treat every request for Protein Science support on a case-by-case basis. We take time to understand the nuances specific to the protein target in question. Then, based upon any literature precedent and our in-house experience, each proposal is tailored to the end use and project goals.

Across our capabilities

Construct Design

• Structure-guided sequence design.
• In-house design and subcloning for rapid turn-around of expression vector preparation/mutagenesis.
• Versatile expression vectors for wide choice of tagging for solubility enhancement, purification and downstream processing with tag cleavage possibility.

Cell Science

• Commercial and in-house modified E. coli strains for well folded proteins.
• Mammalian cell expression in HEK293-6E, CHO-3E7 and HEK Expi293.
• Insect cell expression in Sf9, Sf21, Hi5 cells.
• Expression screening of different conditions for best yield of well folded protein.
• Scale-up expression in the best condition up to 25L scale.
• 15N, 13C, 2H protein labelling in minimal media to support NMR.
• Low endotoxin batches.

Protein Purification

• Standard chromatography methods for purification of high grade protein.
• In vivo biotinylation of target protein.
• Dedicated Membrane protein production team.
• Protein refolding from inclusion bodies.
• Low endotoxin batches.

Protein Analysis Quality Control

• Standard analytical package typically includes SDS-PAGE, Analytical SEC, A280, intact mass spectrometry.
• SPR / DLS / MST / FTSA / CETSA available through our Biophysics team.
• Also available
  • Peptide mapping / LCMS
  • Endotoxin LAL assay
  • SEC-MALS
  • FIDA
  • ITC
  • SEC-MALS & FSEC